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. 2019 Oct 29;10:2417. doi: 10.3389/fmicb.2019.02417

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Copyright © 2019 Rizvi, Shankar, Chatterjee, More, Bose, Dutta, Balakrishnan, Madugulla, Rapole, Mande, Banerjee and Mande.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ammonia release and alleviation of stress. (A) Ammonia estimation post stress in the respective culture media supernatant using Nessler’s assay as assessed by absorbance at 430 nm normalized to respective blank; (B) Measurement of change in pH in M.tb culture post stresses; and (C) urease activity (in terms of ammonia nmol/mg protein/min) in M.tb cell lysates post stress measured by using urease activity assay kit (ab204697, Abcam Plc., United Kingdom). ^∗∗Indicated p-value < 0.005 and ^∗ indicated p-value < 0.05 as compared to control. # ND, not detectable.