. 2019 Nov 4;9:15972. doi: 10.1038/s41598-019-52340-0

Table 1.

Initial rates of cleavage and extent digestion of dU49 by UDG/APE1.

[Mg2+] (mM)	Substrate	k (% cleaved per minute)
0.2 mM	12-601-dU	24.8 ± 2.6
	1-NCP-dU	4.6 ± 0.3^a
	12-NCP-dU	4.7 ± 0.8^a
	1-NCP(18ac)-dU	5.6 ± 0.8^a
	12-NCP(18ac)-dU	6.4 ± 0.4^a
	1-NCP(27ac)-dU	2.4 ± 0.5^a,b
	12-NCP(27ac)-dU	1.5 ± 0.6^a,b
2.0 mM	12-601-dU	86.2 ± 9.8
	1-NCP-dU	8.2 ± 0.8^a
	12-NCP-dU	6.8 ± 0.8^a
	1-NCP(18ac)-dU	8.2 ± 1.4^a
	12-NCP(18ac)-dU	6.9 ± 1.0^a
	1-NCP(27ac)-dU	9.1 ± 1.0^a
	12-NCP(27ac)-dU	6.0 ± 0.9^a

Initial rates were calculated by fitting the digestion data in Fig. 3 to a single phase exponential and multiplying each rate constant by the Y_max as previously described^12,63. ^aP < 0.05 compared to naked DNA. ^bP < 0.05 compared to non-acetylated substrate.