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. 2019 Nov 5;7:34. doi: 10.1038/s41413-019-0070-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Osteocyte-specific reduction in MMP13 expression in the cortical and subchondral bone causes increased trabecular bone mass. a–d In cortical bone (a) imaged at low (left and center, arrows indicate periosteal surface, scale bars: 50 μm) and high (right, scale bars: 10 μm) magnification and in subchondral trabecular bone (b, scale bars: 50 μm), the number of osteocytes stained positive for MMP13 is reduced in MMP13^ocy−/− bone compared with wild-type (c, d, n = 6-9). No differences were observed in osteoblasts in the periosteum, in bone marrow, or in negative control images. e MMP13 mRNA was also significantly reduced in MMP13^ocy−/− bone compared with wild-type (n = 7–9). f Osteocyte number was not changed between groups. g MMP13 expression in chondrocytes was not significantly different between groups (scale bars: 50 μm, n = 6–8). h μCT of distal femoral trabecular bone (n = 11) shows that bone volume fraction and volumetric bone mineral density are increased in MMP13^ocy−/− bone due to an increase in the trabecular number and corresponding decrease in trabecular spacing rather than a change in trabecular thickness. Decreased SMI indicates a shift to more plate-like trabecular structures. i Cortical bone, however, did not present differences in thickness or mineral density. Graphs show mean ± SD. *P < 0.05 between genotypes by unpaired t test