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. 2019 Nov 4;9:15904. doi: 10.1038/s41598-019-52347-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Release of soluble components in pretreatment filtrate from raw and PSCB. (a) Release of % SRS in pretreatment filtrate from raw untreated control, NaOH, NH₄OH and H₂SO₄ pretreatments; Significance difference between 1.25% (), 2.5% () and 5.0% () SCB biomass loading for pretreatments is given as *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 for Sidek test-Two-way ANOVA analysis; Values presented are Mean ± Standard Errors of the Mean (SEM), for n = 3. (b) Representative chromatographs of HPLC analysis for pretreatment filtrates of 5% (w/v) loading of SCB for Raw untreated control, 3% NaOH, 3% NH₄OH and 3% H₂SO₄ pretreatment; RT: Glucose ~11.4 min, Xylose ~12.1 min; Dashed line () at X-axis indicates the time span of 5 min on chromatogram; n = 3.

Inline graphic — Release of soluble components in pretreatment filtrate from raw and PSCB. (a) Release of % SRS in pretreatment filtrate from raw untreated control, NaOH, NH₄OH and H₂SO₄ pretreatments; Significance difference between 1.25% (), 2.5% () and 5.0% () SCB biomass loading for pretreatments is given as *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 for Sidek test-Two-way ANOVA analysis; Values presented are Mean ± Standard Errors of the Mean (SEM), for n = 3. (b) Representative chromatographs of HPLC analysis for pretreatment filtrates of 5% (w/v) loading of SCB for Raw untreated control, 3% NaOH, 3% NH₄OH and 3% H₂SO₄ pretreatment; RT: Glucose ~11.4 min, Xylose ~12.1 min; Dashed line () at X-axis indicates the time span of 5 min on chromatogram; n = 3.