Figure 1.

Spatio-temporal dynamics of thrombus formation in inflamed venular microvessels. Non-perfused arterioles, capillaries, and venules were quantified in the cremaster muscle of unstimulated control animals and in animals receiving an intrascrotal injection of LPS as well as intravenously neutrophil-depleting anti-Ly-6G mABs or isotype control Abs (A; mean ± SEM for n = 4 per group; *p < 0.05 vs. control, ^#p < 0.05 vs. neutrophil depletion). Thrombus formation in postcapillary venules of the mouse cremaster muscle was induced by photochemical injury as detailed in Methods, representative in vivo fluorescence microscopy images of time-lapse video recordings are shown (B; scale bar: 20 μm, Video S1, 4). Panels show quantitative data for onset and cessation times in WT mice receiving a local, intrascrotal injection of PBS (‘unstimulated’) or LPS (‘inflamed’) (C,D; mean ± SEM for n = 9 per group; *p < 0.05 vs. unstimulated control) and undergoing treatment with heparin, platelet-depleting antibodies, or vehicle/isotype control antibodies (E,F; mean ± SEM for n = 3–4 per group; *p < 0.05 vs. vehicle/isotype control). Aggregation patterns of fluorescence-labeled platelets during thrombus formation in unstimulated or inflamed capillary and venular cremasteric vessels were visualized by multi-channel in vivo fluorescence microscopy as detailed in Methods, representative images of time lapse video recordings are shown (G; platelets in white, scale bar: 40 μm, Video S2, 3).