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. 2019 Nov 4;9:15932. doi: 10.1038/s41598-019-52041-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Role of neutrophils for thrombus formation in inflamed venular microvessels. Intravascular adherence of leukocytes was analyzed in postcapillary venules of the cremaster muscle of WT mice receiving an intrascrotal injection of PBS (‘unstimulated’, grey plot) or LPS (‘inflamed’, green plot) by RLOT in vivo microscopy as detailed in Materials and Methods, representative images are shown (A; scale bar: 40 μm). Panel (B) shows quantitative data for leukocyte intravascular adherence in venules (mean ± SEM for n = 4 per group; *p < 0.05 vs. unstimulated control). Thrombus formation in postcapillary venules of the cremaster muscle of WT mice was induced by photochemical injury as detailed in Methods. Panel (C) shows quantitative data for cessation times in WT mice undergoing intrascrotal stimulation with LPS after treatment with neutrophil-depleting or isotype control antibodies (mean ± SEM for n = 4 per group; *p < 0.05 vs. isotype control). Interactions of platelets (white) and neutrophils (orange) during thrombus formation in postcapillary venules of the cremaster muscle of WT mice induced by photochemical injury were visualized by multi-channel in vivo fluorescence microscopy as detailed in Methods, representative images are shown (D; arrow indicates flow direction, scale bar: 20 μm, Video S5, 6).