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. 2019 Nov 4;9:15876. doi: 10.1038/s41598-019-52456-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In toto immunolocalization of GST during PSCs oxidative stress response. PSCs were incubated in RPMI-10% FBS (C-PSCs) (A to H) or RPMI-10% FBS supplemented with 2.5 mM H₂O₂ (H-PSCs) (I to L). After PFA fixing, PSCs were incubated with anti-GST antibody (G and K) (GST) (green) and counterstained with DAPI-phalloidin (DAPI/PHAL) (see Materials and Methods section). Negative control consisted of omission of primary antibody (C). A, E, and I show the bright field and merge of the three channels in D, H and L. Scale bar = 20 μm. P: parenchyma: S: sucker: Tg: tegument.