Fig. 2. GATA3 is physically associated with UTX/MLL4 complex.

a, b Association of UTX with GATA3 in MCF-7 and T-47D cells. Whole-cell lysates were prepared, and co-IP was performed using antibodies against GATA family or UTX, and then captured samples were immunoblotted with antibodies against the indicated proteins. IgG served as the negative control. c Association of GATA3 with MLL3/MLL4 complex in MCF-7 cells. Whole-cell lysates were immunoprecipitated with antibodies against GATA3, MLL3, or MLL4-complex proteins and immunocomplexes were immunoblotted with antibodies against the indicated proteins. d Interaction between GATA3 and UTX is independent of ERα. Whole-cell lysates were prepared from MCF-7 cells and co-IP was performed using antibodies against GATA3 or UTX, after which IB was performed with antibodies against the indicated proteins to examine the interaction in the presence and absence of ERα