Fig. 3. Molecular interaction between GATA3 and UTX/MLL4 complex.

a GST pull-down experiments were performed using bacterially expressed GST-fusion proteins and proteins prepared using in vitro transcription/translation, as indicated. b GATA3 directly interacts with JmjC domain of UTX in vitro. GST pull-down assays were performed using a series of truncation constructs of UTX and in vitro transcribed/translated GATA3. c GATA3 directly interacts with PHD-WH domain of ASH2L in vitro. GST pull-down assays were conducted using a series of truncation constructs of ASH2L and in vitro transcribed/translated GATA3. d GATA3 directly interacts with N-terminal region of RBBP5 in vitro. GST pull-down assays were performed using a series of truncation constructs of RBBP5 and in vitro transcribed/translated GATA3. e Identification of GATA3 domains required for interaction with UTX, ASH2L, or RBBP5. GST pull-down assays were conducted using a series of truncation constructs of GATA3 and in vitro transcribed/translated proteins, as indicated. f Schematic depiction of molecular interactions between GATA3 and MLL4 complex