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. 2019 Nov 4;9:15942. doi: 10.1038/s41598-019-52286-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scheme of proliferation and/or inhibition of PBMCs assays. PBMCs from the study subjects were cultured with different combinations of peptides or/and olive-pollen extract (Olea) as indicate the figure, for 6 days, in order to determine: the proliferative response (a), reversion of the response (b) or inhibition response (c). Three different combinations of peptides were used: peptides 2 and 3 (immunoregulatory region); peptides 10, 12, and 13 (immunodominant region), and both groups of peptides. After 6 days of stimulation, proliferative response was measured. Also, supernatants (SN) were collected for a subsequent study of cytokine secretion.