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. 2019 Nov 4;9:15903. doi: 10.1038/s41598-019-52383-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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¹H-detected REDOR dephasing curves, using a selectively Leu/Val ¹³CHD₂ methyl labelled and a 5% GlcRAP labelled sample of α-spectrin SH3. The external magnetic field was 14.1 T (600 MHz). The effective temperature of the sample was adjusted to 20–25 °C. The MAS frequency was set to 50 kHz. In the experiment, a ζ-delay of 4 µs was employed. The methyl order parameter S² (S²_axis in Eq. (4)) and the asymmetry η were determined as described in the Methods section. The values are given at the bottom of each plot. In the first two rows, the dephasing curves were fitted by simultaneously minimizing χ² for both methyl sites (γ1/γ2 for Val and δ1/δ2 for Leu) to improve the fitting convergence. This yielded one order and asymmetry parameter for each Val and Leu residue. However, individual fits for γ1/γ2 (Val) and δ1/δ2 (Leu) were plotted in red and blue to indicate that proR and proS methyl groups show very similar dynamics. The figure was generated with Adobe Illustrator CS5 V.15.02.