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. 2019 Nov 4;9:15903. doi: 10.1038/s41598-019-52383-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Dipolar coupling anisotropies δ_aniso (top) and asymmetries η (bottom), using the 5% GlcRAP (red) and the selectively Leu/Val ¹³CHD₂ methyl labelled sample (blue) of α-spectrin SH3, respectively. All values were determined by fitting REDOR dephasing curves represented in Fig. 4. (B) The anisotropies and asymmetries of both samples are linearly correlated, setting the slope to 1.0 and the y-intercept to 0.0. The linear correlation coefficient R² is 0.96 and 0.85 for δ_aniso and η, respectively. The figure was generated with Adobe Illustrator CS5 V.15.02.