Table 2.
Properties of the rhamnosidases analyzed in this study.
| Protein | strain | MW (kDa) | theor. PI | fold change in activity with CoCl2a | specific acitivtya,b (U mg−1) | Temp.Opt. (°C) | pH 60% activity range | Km or Kprime, hb,c (mM) | kcatb (s−1) | Kib, αb (mM rhamnose) | IC50 (at Km or Kprime) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Tm_Ram106B | Thermotoga maritima MSB8 | 116.6 | 5.95 | 25.1 | 40.5 ± 0.33 | 86.5 | 5.5–7 | 1.94 ± 0.20 | 217.8 ± 10.1 | 2.37 ± 0.13 | 4.9 |
| Tn_Ram106B | Thermotoga neapolitana Z2706-MC24 | 115.8 | 5.89 | 60.8 | 32.23 ± 0.19 | 84 | 5–6.6 | 1.47 ± 0.14 | 146.8 ± 6.1 | 5.50 ± 1.01, 1.40 ± 0.44 | 6.5 |
| Cb_Ram106B | Caldicellulosiruptor bescii DSM 6725 | 115.9 | 6.31 | 52.9 | 7.38 ± 0.21 | 70 | 5–6.6 | 1.29 ± 0.23, 1.64 ± 0.20 | 51.9 ± 6.0 | — | 5.5 |
| Ts_Ram106B | Thermoclostridium stercorarium subsp. stercorarium DSM 8532 | 121.3 | 5.63 | 6.9 | 2.13 ± 0.01 | 50 | 5–5.6 | 1.90 ± 0.18 | 14.3 ± 0.5 | 4.94 ± 0.75, 2.56 ± 0.63 | 8.3 |
aOn 1 mM pNPR with 1 mM CoCl2.
b±standard deviation.
cKprime/hill-coefficient h for Cb_Ram106B.