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. 2019 Oct 29;10:1344. doi: 10.3389/fphys.2019.01344

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Copyright © 2019 Lopalco, Vitale, Cho, Totaro, Corcelli and Lobasso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of TLC lipid profiles of semen, seminal plasma and spermatozoa. Total lipids were extracted from whole semen (S) as well as from related spermatozoa (Sz) and seminal plasma (Sp) obtained from a man with normozoospermia and analyzed by TLC with the same solvent, as described in Methods. All species of lipids are stained by iodine vapor (A); sulfur-containing lipids are stained by azure A reagent (B). Lipid bands are indicated by their abbreviations: phosphatidylinositol (PI), lysophosphatidylcholine (LPC), sphingomyelin (SM), phosphatidylserine (PS), phosphatidylcholine (PC), phosphatidylethanolamine (PE), cardiolipin (CL), cholesterol (Chol), cholesterol sulfate (Chol-S), and seminolipid (SGG).