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. 2019 Oct 29;10:1344. doi: 10.3389/fphys.2019.01344

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Copyright © 2019 Lopalco, Vitale, Cho, Totaro, Corcelli and Lobasso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Negative ion mode MALDI-TOF/MS analyses of the lipid extracts of semen, seminal plasma and spermatozoa. Total lipids were extracted from whole semen (S) as well as from related spermatozoa (Sz) and seminal plasma (Sp) obtained from a man with normozoospermia. In panel (A), the typical lipid profiles are shown. The main peaks are attributable to Chol-S [M–H]^– at m/z 465.3 and SGG O-32:0 [M–H]^– at m/z 795.7. In panel (B), the enlargement of the region at m/z 760–840 of mass spectrum of S is shown. The peaks at m/z 767.6, 809.7, 821.7, and 823.7 are assigned to other SGG species (O-30:0, O-33:0, O-34:1 or P-34:0, and O-34:0, respectively); their molecular structures are also drawn.