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. 2019 Oct 29;10:1344. doi: 10.3389/fphys.2019.01344

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Copyright © 2019 Lopalco, Vitale, Cho, Totaro, Corcelli and Lobasso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Negative ion mode MALDI-TOF/MS analyses of the lipid extracts of semen of normozoospermic subjects and oligoasthenozoospermic patients. In panel (A), two typical lipid profiles are shown. The histograms show the significant differences in intensity of lipid peaks at m/z 465.3 and 795.7 corresponding to Chol-S and SGG O-32:0 (B), at m/z 767.6, 809.7 and 823.7 corresponding to main SGG species (C), at m/z 716.6, 744.6 and 778.7 corresponding to PE species (D). A p-value from Student’s t-test <0.05 was set as the threshold to define significant differences between the peaks present in the two series of spectra. Data are reported as the average value ± SD.