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. 2019 Oct 29;10:1344. doi: 10.3389/fphys.2019.01344

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Copyright © 2019 Lopalco, Vitale, Cho, Totaro, Corcelli and Lobasso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of TLC sulfolipid profiles of semen of normozoospermic subjects and oligoasthenozoospermic patients. Total lipids were extracted from semen samples of normozoospermic (a, b, c, d, e) and oligoasthenozoospermic (a′, b′, c′, d′, e′) subjects and then eluted with the same solvent as in TLC showed in Figure 1. Azure A reagent was used to stain sulfur-containing lipids only. Lipid bands assigned to cholesterol sulfate (Chol-S) and seminolipid (SGG) are indicated by their abbreviations.