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. 2019 Oct 29;6:156. doi: 10.3389/fcvm.2019.00156

Figure 1.

Figure 1

Hypoxia promotes expression of matrix remodeling enzymes in aged (>2 years) porcine aortic valves (AVs) in culture. (A) Western blot analysis of tissue lysates from fresh, normoxic (20% O2) and hypoxic (13% O2) porcine AVs cultured for 1 week. (B) Quantification of western blot band intensities using densitometry and normalized to βActin. Data shown are mean ± SD from N = 3 animals. *P < 0.05, **P < 0.01, ***P < 0.001 vs. fresh AV controls, and †P < 0.05 between 20 and 13% O2 cultured AVs. Significance determined by one-way ANOVA using Tukey post-hoc test. MMP9-NGAL and NGAL-MMP9 represent bands for the complex detected when probed separately for MMP9 and NGAL, respectively.