THSD1 is required for normal levels of focal adhesions and THSD1 loss reduces the overall amount of focal adhesions, FAK, and active phosphorylated FAK in endothelial cells. As a result, several pathways may be implicated including SRC, PI3K/AKT, Rho, and Rac1 signaling that affects actin cytoskeleton organization and cell adhesion mediated in part through integrins and mechanosensors.