Figure 4.

PpV mutants frequently undergo an extra nuclear cycle. (A) Images from time-lapse movies (movie 2) of wild type and PpV embryos expressing Histone2Av-RFP. Examples of PpV embryos with a normal number of nuclear cycles, a partial and a complete extra nuclear cycle are shown. Time is indicated in minutes:seconds. Scale bar 50 μm. (B) Quantifications for cell cycle lengths and (C) nuclear density. PpV embryos were grouped in ones with normal number of nuclear cycle (13 div.) and ones with extra cycle (14 div.). Rectangles indicate mean values. Error bars indicate standard deviations. P > 0.05, not significant (ns); P < 0.0005 (***). Statistical significance was calculated by Student’s t-test. Source data are listed in Table S1.