Figure 5.

Fc-VEGFR2 binds to VEGF-Fn in a VEGF-Dependent manner. Fn (fibronectin) was adsorbed onto polystyrene plates overnight at 4 °C in the presence of heparin. The wells were washed to remove the heparin and incubated with the indicated VEGF concentrations. Unbound VEGF was removed prior to addition of 25 ng Fc-VEGFR1 (open circles) or Fc-VEGFR2 (closed circles) for 1 h at room temperature. The wells were washed and the Fc-chimera proteins were detected with HRP labeled goat anti human IgG. The absorbance values were determined in quadruplicate wells and the data is expressed as the average (N = 4) percentage relative to the absorbance observed with each chimera when no VEGF was present (zero VEGF was set to 100% for each chimera) ± SEM.