Figure 9.

Schematic of key methods. On the left is the general cell attachment protocol. Fluorescent RECs were allowed to attach to RPEs that had been preconditioned ± hypoxia. In some studies, soluble antagonists were added with the RECs and in others the cells were treated prior to REC attachment (i.e., with VEGF or heparinase III). On the right is the general approach used to evaluate VEGF-receptor binding to RPEs and Fn, as well as the method used to analyze HS levels on RPEs.