Skip to main content
. 2019 Oct 22;20(20):5240. doi: 10.3390/ijms20205240

Figure 2.

Figure 2

The fusTE is not involved in producing fusaricidins. Organization of the eight genes within the fus gene cluster in P. polymyxa WLY78 was determined by RT-PCR. The numbers on the top of the gels correspond to the product numbers drawn schematically in the outline given above. RT is the standard RT-PCR reaction; (–) is the negative control in which no reverse transcriptase was added to the RT reaction; (+) is the positive control in which genomic DNA was used as the template in the RT reaction (A). Antifungal activity of fus gene mutants (fusA−, ΔfusB, ΔfusC, ΔfusD, ΔfusE, ΔfusF, ΔfusG, and ΔfusTE) against F. oxysporum f. sp. cucumerium (B). Collection of fusaricidins extracted from wild-type WLY78 (1), ΔfusTE (2), fusA− (3), ΔfusB (4), ΔfusC (5), ΔfusD (6), ΔfusE (7), ΔfusF (8), and ΔfusG (9) cells, according to the elution time between 18.7 min and 20.6 min (C), and then each fraction was bio-assayed against F. oxysporum f. sp. cucumerium (D).