Figure 5.

Validation of matrisome proteins in the in vivo wound bed in a murine wound healing model. (a) In wounds that were harvested three days post wounding the macrophage-specific F4/80 as well as transforming growth factor beta induced (TGFBI), MMP9 and EMILIN-1 were detected by immunofluorescence analysis within the wound (a’) or the intact skin (a´´). (b) TGFBI, MMP9, EMILIN-1, netrin-1 and gliomedin abundance (arrowhead) was analyzed by immunoblotting in lysates of intact skin (skin) or wounds three (D3) and five days post wounding (D5). Actin was used as loading control. As EMILIN-1 and gliomedin were tested on a single blot, the same actin control is included in both panels. Molecular weights of Thermo Scientific™ PageRuler™ Plus Prestained 10–250 kDa Protein Ladder bands are given. *, unspecific band; scale bars (a), 1000 µm; (a´, a´´), 200 µm.