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. 2019 Oct 11;20(20):5033. doi: 10.3390/ijms20205033

Figure 2.

Figure 2

Nedd4-2-dependent regulation of sodium current is abrogated by the SCN5A-p.Y1977N mutation in HEK293 cells. Typical example of sodium current recordings obtained from HEK293 cells transiently transfected with SCN5A wild-type (WT) (A) or SCN5A-p.Y1977N (YN) (B) with or without Nedd4-2. Average sodium current-voltage relationship (C) and voltage dependence of activation and inactivation (D) for HEK293 cells transiently transfected with SCN5A WT or YN, in the presence and absence of Nedd4-2. n indicates the number of cells. Insets: voltage clamp protocols. * p < 0.05 vs. WT, # p < 0.05 vs. YN + Nedd4-2; two-way repeated measures ANOVA followed by Holm–Sidak test for post-hoc analysis.