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. 2019 Oct 11;20(20):5033. doi: 10.3390/ijms20205033

Figure 3.

Figure 3

The SCN5A-p.Y1977N mutation abolishes the Nedd4-2 mediated decrease of Nav1.5 cell surface expression. (A) HEK293 cells were transiently transfected with the indicated plasmids and whole cell lysates were blotted against Nav1.5, Nedd4-2 or actin. (B) Fluorescence intensity of Nav1.5 wild-type (WT) and Y1977N (YN) with or without Nedd4-2, was quantified with LICOR Odyssey software. Data are expressed as fold change compared to the WT or YN group. (* p < 0.05, Unpaired Student’s t-test; n corresponds to the number of experiments performed). (C) Western blot of a representative cell surface biotinylation assay in HEK293 cells transiently expressing Nav1.5 WT or YN with or without Nedd4-2. (D) Quantification of Nav1.5 protein at the cell surface in the different conditions described in panel C using LICOR Odyssey software. Data are expressed as fold change compared to the WT or YN group. (* p < 0.05, Unpaired Student’s t-test; n corresponds to the number of experiments performed).