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. 2019 Sep 27;8(10):1163. doi: 10.3390/cells8101163

Figure 5.

Figure 5

Effects of KA on Janus kinase/signal transducers and activators of transcription (JAK/STAT) phosphorylation in LPS-induced RAW 264.7 macrophages. (A,B) Cells were pretreated with KA (20, 40, or 80 μM) for 1 h, and then stimulated with LPS (1 μg/mL) for 120 min (STATs) or 60 min (JAKs). Total cellular proteins were prepared, resolved by SDS-PAGE, and detected using specific p-STAT1 (Y701 and S727), p-STAT3 (Y705), STAT1, STAT3, p-JAK1 (Y1022), JAK1, p-JAK2 (Y1007/1008), and JAK2 antibodies. β-actin was used as an internal control. Each experiment was performed three times, and similar results were obtained in each experiment. # P < 0.05 vs. the control group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. LPS-stimulated cells.