Skip to main content
. 2019 Sep 27;8(10):1163. doi: 10.3390/cells8101163

Figure 7.

Figure 7

Effects of KA on histological changes and the expression of tight junction (TJ)- and epithelial–mesenchymal transition (EMT)-related markers in DSS-induced colonic tissues. (A) Representative sections of colon tissues from mice administrated with 4% DSS in drinking water for 7 days with KA (5 or 20 mg/kg/day, i.p.). 5-ASA (75 mg/kg/day, p.o.) was used as a positive control. Histological changes were determined by H&E staining. (B–D) Total RNAs were prepared from DSS-induced colon tissues and analyzed for the mRNA expression of F4/80, Ly6G, zona occludens (ZO-1), occludin, claudin1, E-cadherin, N-cadherin, and vimentin by qRT-PCR. The levels of F4/80, Ly6G, ZO-1, occludin, claudin1, E-cadherin, N-cadherin, and vimentin were adjusted by β-actin expression. Values are the mean ± SDs (n = 12); # P < 0.05 vs. the vehicle-treated control group; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. the DSS-treated group.