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. 2019 Oct 9;20(20):4983. doi: 10.3390/ijms20204983

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MAT1A overexpression decreases cell proliferation and transcriptional activity. (A) Proliferation indices over 72 h (* p < 0.05) in 5637 and 5637^MAT1A⁺ cells and (B) percentage of 5637 versus 5637^MAT1A⁺ cells in various generations following initial cell seeding at generation zero (* p < 0.05). Cells were stained with carboxyfluorescein diacetate succinimidyl ester (CFSE) and analyzed using flow cytometry to evaluate changes in proliferation. Data was analyzed and proliferation was modeled in FlowJo^®. RNA-seq analysis identified whole genome transcriptomic alterations in 5637^MAT1A⁺ and 5637 cells. Heat maps representing differentially expressed genes (n = 3 biological replicates) reveal downregulation in three gene groups: (C) zinc finger, (D) histone, and (E) transcription factors.