AChRs are upregulated in active zones of infiltration in GBM patients. mRNA expression of CHRNA7, CHRNB2, CHRM1, and CHRM3 within cellular tumor (CT), infiltrating tumor (IT), and leading edge (LE) populations from GBM samples in the Ivy GAP dataset. Tukey’s honest significant difference test was used to compare the expression changes in the IT and LE populations in comparison to the expression within the CT population. CHRNA7: CT vs. LE p < 0.01 **, CT vs. IT p < 0.05 *; CHRNB2: CT vs. LE p < 0.001 ***, CT vs. IT p < 0.05*; CHRM1: CT vs. LE p < 0.001 ***, CT vs. IT p < 0.001 ***; CHRM3: CT vs. LE p < 0.001 ***, CT vs. IT p = 0.21.