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. 2019 Oct 17;20(20):5150. doi: 10.3390/ijms20205150

Figure 5.

Figure 5

WECS increases mitochondrial biogenesis and oxygen consumption. Dose-dependent effects of WECS on UCP1 expression in brown adipocytes (A). Thermogenic and mitochondrial biogenic gene expression in brown adipocytes treated with 1 μg/mL WECS or DMSO (B). The expression levels of adipogenic marker genes (C). The mitochondrial copy numbers (D). Representative images of UCP1 staining (E). The protein levels of UCP1 and OXPHOS in brown adipocyte (F). Brown adipose primary cells were treated with 1 μg/mL WECS or DMSO during 6 days of brown adipogenesis; OCR at day 6 (G). The OCR-related basal metabolic rate, ATP levels, maximum oxygen consumption, and proton leakage, were all significantly increased (H). Bars represent the mean + SEM, n = 3–5. * p < 0.05, ** p < 0.01 compared with the HFD control.