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. 2019 Oct 16;20(20):5118. doi: 10.3390/ijms20205118

Figure 2.

Figure 2

Human lncRNA ISR is induced by several virus infections. (a) The lncRNA ISR and IFN-β expression levels in indicated human cell lines infected with or without IAV WSN (MOI = 0.8) for 16 h was examined by RT-PCR; (b) A549 cells were infected with IAV WSN (MOI = 0.4) for indicated times. RT-PCR was performed to determine lncRNA ISR expression; (c–g) The lncRNA ISR and IFN-β expression levels were examined in A549 cells infected with IAV PR8 (c), IAV CA04 (d), HSV (e), SeV (f), PRV (g) by RT-PCR and qRT-PCR. Data are represented as mean ± S.D. ** p < 0.01; *** p < 0.001. A549 cells were transducted with pseudovirus (LentV) prepared by lentivirus expression system (h), or incubated with lipopolysaccharide (LPS) for 8 h (i), or cultured in serum-free media for indicated time (j). The expression of lncRNA ISR were determined by RT-PCR.