Figure 5.

LncRNA ISR expression is induced by IFN-β. (a,b) A549 cells were treated with IFN-β at the indicated concentrations for 3 h. After treatment, total RNA was extracted for RT-PCR (a) and qRT-PCR (b) to detect lncRNA ISR expression. Data are represented as mean ± S.D. * p < 0.05; ** p < 0.01; (c,d) Mouse cells (4T1 cells, RAW264.7 cells, NIH/3T3 cells) were treated with IFN-β at indicated concentrations for 3 h. RT-PCR (c) and qRT-PCR (d) was performed to determine the lncRNA ISR expression. Data are represented as mean ± S.D. * p < 0.05; ** p < 0.01; (e,f) C57BL/6 WT and IFNAR1-KO mice were infected with or without 5 × 10⁴ pfu of IAV WSN virus (n = 10 mice/group). At 16 hpi, total RNA was extracted for RT-PCR (e) and qRT-PCR (f) to detect lncRNA ISR expression. Data are represented as mean ± S.D. * p < 0.05.