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. 2019 Oct 17;20(20):5141. doi: 10.3390/ijms20205141

Figure 3.

Figure 3

Analysis of osteoclast differentiation of primary bone-marrow macrophages. Increased and enlarged osteoclasts positive for TRAP staining occurred in glucocorticoid-treated WT mice, whereas few osteoclasts formed in miR-29aTg mice (A) scale bar, 8 μm. miR-29a overexpression repressed the glucocorticoid-induced increases in osteoclast number and area (B) and also reduced osteoclastogenic markers NFATc1, cathepsin K (C), and osteoclast maturation markers carbonic anhydrase II and V-ATPase expression (D). Data are expressed as the mean ± standard errors calculated from 6 mice. Asterisks * indicate significant differences from the WT + Veh group and hashtags # indicate significant differences from the WT + Veh group (p < 0.05). WT, wild-type mice; Tg, miR-29aTg mice; Veh, vehicle; GC, glucocorticoid, Oc, osteoclasts; Oc.Ar, osteoclast area; NFATc1, nuclear factor of activated T-cells-c1; Ca II, carbonic anhydrase II; V-ATPase, vacuolar H+-ATPase.