Skip to main content
. 2019 Oct 21;20(20):5206. doi: 10.3390/ijms20205206

Figure 2.

Figure 2

MALDI-TOF MS spectra of N-glycans on glycoprotein E of purified ZIKV. N-linked glycans were prepared from envelope protein(s) of Zika viruses (A) MR766, (B) PRVABC59, (C) FLR, (D) IbH, and (E) SJRP were produced from various cell types (C6/36, LLC-MK2, Vero, THP-1, SNB-19, and JEG-3 cells) and identified by MALDI-TOF mass spectrometry. The virions were purified using sucrose cushion ultracentrifugation and resolved on SDS-PAGE (see Figure S1). The presence of glycoprotein E was confirmed by Western blotting. The corresponding glycoprotein E band from mass spectrometry compatible Coomassie stained gel was excised and subjected to N-glycan release. The N-linked glycans were identified using MALDI-TOF mass spectrometry. X axis is mass to charge ratio (m/z) and Y axis represents signal intensity of the ions. Schematic representation of the N-linked glycans (high-mannose, hybrid and complex) and the corresponding molecular ions that were predicted to be identified in this study. Yellow circle, galactose; blue square, N-acetylglucosamine; green circle, mannose; red triangle, fucose; purple diamond, sialic acid.