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. 2019 Oct 21;20(20):5206. doi: 10.3390/ijms20205206

Figure 6.

Figure 6

The functional importance of N-linked glycans on Zika virion infectivity. (A) DC-SIGN/L-SIGN-mediated enhancement of Zika virus infection. 3T3, 3T3-DC-SIGN, and 3T3-L-SIGN cells were infected with ZIKV at MOI of 1, and the expression of viral E glycoprotein was detected 24 h later using an immunofluorescence assay. (B) DC-SIGN/L-SIGN-mediated enhancement of Zika virus infection is inhibited by Mannan. 3T3-DC-SIGN or 3T3-L-SIGN cell lines were incubated with Mannan (at 200 μg/mL) inhibitors for 30 min at 37 °C and then infected with ZIKV at MOI of 1. The same concentration was maintained constant throughout duration of infection. (C) DC-SIGN/L-SIGN-mediated Zika virus infection is reduced by EDTA. 3T3-DC-SIGN or 3T3-L-SIGN cell lines were pre-incubated with EDTA (at 0.5 mM) for 30 min at 37 °C and then infected with ZIKV at MOI of 1. The treatment was continued for 2 h. After 2 h, the virus and the EDTA-containing medium were removed and the infection was continued with fresh medium. After 24 h, the infected cells were detected using viral E protein antibody and the percent of infected cells was assessed by an immunofluorescence assay using Operetta High-Content Imaging System. Error bars indicate standard deviations calculated from four replicate wells in a 96-well plate.