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. 2019 Oct 9;20(20):4985. doi: 10.3390/ijms20204985

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Preparation of 5/6-nephrectomized rats and the effect of ZnSO₄ injection on their red blood cell (RBC) levels. (A) Normal rats underwent a sham-operation or surgical removal of 5/6 of the kidney. After 25 days, rat blood was drawn, and RBC levels and other hematological indices of the rats were measured. (B) Effect of ZnSO₄ injection on the RBC level of 5/6-nephrectomized rats. The 5/6-nephrectomized rats (25 days post surgery) were injected with either saline or 2.8 mg Zn/kg body weight of ZnSO₄ solution. One, 2, or 4 days after injection, RBC levels and other hematological indices were measured. ** Significant difference between ZnSO₄-injected and saline-injected rats (p < 0.01, n = 6). This figure is representative of three independent experiments.