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. 2019 Oct 9;20(20):4985. doi: 10.3390/ijms20204985

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Comparison between the injection of saline, ZnSO₄, or rHuEPO on the RBC level of 5/6-nephrectomized and normal rats. (A) Injection of saline, ZnSO₄, or rHuEPO on the RBC levels of 5/6-nephrectomized rats. Rat blood was drawn from the 5/6-nephrectomized rats (25 days post surgery); then, the rats were injected with saline, ZnSO₄, or rHuEPO. Every 7 days, blood was drawn, and saline, ZnSO₄, or rHuEPO was injected. The collected blood was used to measure RBC levels and other hematological indices. * and **: Significant difference between ZnSO₄- or rHuEPO- and saline-injected rats (* p < 0.05; ** p < 0.01; n = 6). (B) Injection of saline, ZnSO₄, or rHuEPO on the RBC level of normal rats. Normal rats (same age and lot as the 5/6-nephrectomized rats) were subjected to the same procedure described above. This figure is representative of three independent experiments.