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. 2019 Sep 20;8(10):1112. doi: 10.3390/cells8101112

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The influence of HtrA4 on viability of cancer cells assayed by AO/EB double staining. The analysis of live (green) and apoptotic cells with concentrated and asymmetrically localized orange-red nuclei was performed using fluorescence microscopy. The A549 cells with exogenous HtrA4 and ΔN-HtrA4 induced by doxycycline were tested.