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. 2019 Oct 15;20(20):5105. doi: 10.3390/ijms20205105

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Staining of α-actinin, Ki-67, and cell nuclei to document cell alignment and proliferation of iPSC-CMs on I. labyrinthus scaffolds. Left panel shows fluorescence channels of α-actinin (green), Ki-67 (magenta), and nuclei (blue), middle panel represents brightfield image, and right panel overlay of brightfield and fluorescence channels. (A) iPSC-CMs on Geltrex^®-coated scaffold representing sarcomere structures of cells attached to a chitin fiber and the presence of a minor population of Ki-67 positive cells. (B,C) Representative images of iPSC-CMs on Geltrex^®-coated and uncoated sponge scaffolds.