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. 2019 Oct 1;8(10):1185. doi: 10.3390/cells8101185

Figure 1.

Figure 1

Inhibitors of mitochondrial activity increase prostaglandin endoperoxide synthase 2 (Ptgs2) expression in rat cortical astrocytes exposed to pro-inflammatory cytokines. (A,B) The mRNA expression of indicated genes in astrocytes treated for 3 hours with interleukin-1α (IL-1α )(A) or with tumor necrosis factor-α (TNF-α) (B); in both cases, cells were also treated with antimycin, oligomycin, or rotenone, as indicated. (C) Western blot analysis of Ptgs2 expression in astrocytes treated for 4 hours with IL-1α in the presence or absence of rotenone, oligomycin, and antimycin, as indicated. (D) Comparative lipidomic analysis. Concentrations of eicosanoids in supernatants of astrocytes treated for 4 hours with IL-1α in the presence or absence of rotenone, oligomycin, and antimycin, and measured using ultra performance liquid chromatography - tandem mass spectrometry. Note: *, p < 0.05 vs. IL-1α or TNFα. Data represent mean values ± SE of 3 or 4 independent experiments.