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. 2019 Oct 10;20(20):5014. doi: 10.3390/ijms20205014

Figure 3.

Figure 3

Induced TRANSPARENT TESTA GLABRA1 (TTG1) activity transcriptionally promotes the expression of several genes involved in flavonoid biosynthesis, including TTG2, TT8, F3’H, DFR, ANS, UGT79B1, UGT75C1, and 5MAT, and the gene related to trichome formation BLT in the young shoots and expanding true leaves. The ttg1-13 35S:TTG1-GR young shoots and expanding true leaves at 20 days after germination were mock-treated (Mock) or treated with 10 μM dexamethasone (DEX), 5 μM cycloheximide (CYC), or 10 μM DEX plus 5 μM CYC (DEX + CYC). The expression of these genes was determined after 1 or 3 h of treatment using qRT-PCR analyses. The house-keeping gene ACTIN7 was used as the internal control. The expression level of each gene was first calculated relative to ACTIN7, and its expression levels in both Mock and CYC treatments were set to 1. Asterisks indicate significant differences in gene expression in DEX-treated samples compared with their respective controls (two-tailed paired Student’s t test, p ≤ 0.05). Values are means ± SD (n = 3). Error bars denote SD.