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. 2019 Oct 22;20(20):5239. doi: 10.3390/ijms20205239

Figure 6.

Figure 6

Analysis of the sirtuin proteinsactivity and expression. The activity of sirtuin proteins was measured in mitochondria fraction using pan-SIRT assay. The error bars represent standard error of the mean (± SEM) (t-test; * p < 0.05; n = 3) (A). Immunoblotting analysis of SIRT3, SIRT4, SIRT5 was performed in the mitochondrial lysates from each group (B, C). The columns represent the average ratios of relative protein expression in control and irradiated samples after background correction (t-test; * p < 0.05; n = 3) (C). The activity of SIRT1 was compared between irradiated and control samples (t-test; * p < 0.05; n = 4) (D). Immunoblotting analysis of SIRT1 was performed using whole heart lysate. The error bars represent standard error of the mean (t-test; * p < 0.05; n = 4) (E,F). The amount of the total protein was measured by Ponceau S staining for an accurate comparison between the groups. The error bars represent standard error of the mean (± SEM) (t-test; * p < 0.05; n = 3).