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. 2019 Oct 14;20(20):5084. doi: 10.3390/ijms20205084

Figure 2.

Figure 2

Downregulation of PGC-1α in TGF-β-treated HK-2 cells. HK-2 cells were exposed to TGF-β. The protein expression of epithelial-mesenchymal transition (EMT) markers (fibronectin, E-cadherin, vimentin, and α-SMA) (A) and PGC-1α (B) was analyzed by western blotting. Bar graphs show mean PGC-1α, fibronectin, E-cadherin, vimentin, and α-SMA/β-actin expression levels, as measured by densitometry. (C) The mRNA expression levels of PGC-1α in TGF-β-treated HK-2 cells were analyzed by real-time polymerase chain reaction (PCR). # p < 0.05, TGF-β-treated vs. untreated.