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. 2019 Oct 14;20(20):5084. doi: 10.3390/ijms20205084

Figure 5.

Figure 5

PGC-1α-specific TGFβRI regulation. To confirm whether TGFβRI modulation is PGC-1α-specific, PGC-1α was knocked down using PGC-1α siRNA (30 nM, Dharmacon) in HK-2 cells (A) and mock and PGC-1α cells (B). After 48 h of transfection, the cells were treated with TGF-β for 2 h, harvested and analyzed by western blotting with the indicated antibodies (against PGC-1α, TGFβRI, and TGFβRII). # p < 0.05, TGF-β-treated vs. untreated mock cells; * p < 0.05, untreated mock cells vs. PGC-1α cells; § p < 0.05, TGF-β-treated mock cells vs. PGC-1α cells; † p < 0.05, siCon vs. siPGC-1α treatment of untreated PGC-1α cells; ‡ p < 0.05, siCon vs. siPGC-1α treatment of TGF-β-treated PGC-1α cells.