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. 2019 Oct 7;8(10):1212. doi: 10.3390/cells8101212

Figure 2.

Figure 2

Consequences of LSD1 inhibition/silencing in human microvascular endothelial cells on checkpoint kinase 1 (Chk1) activation. Immunofluorescent analysis of the Chk1 level (A,B) and the Chk1 activation (ph-Chk1(S317) level (A,C). Cells were stained with anti-Chk1 or anti-ph-Chk1(S317) antibodies conjugated with AlexaFluor 488 fluorescent dye. The labeling index was calculated as the ratio of immunofluorescence-labeled cells to all cells in an HMEC-1 cell population. The intensity of fluorescence was visualized in ImageJ program (B a6, a7, b6, b7; C a6, a7, b6, b7). The presented pictures are representative of three independent experiments. (D), (E) Analysis at the protein level of the expression of Chk1 and ph-Chk1 in (D) pharmacological and (E) transcriptional experimental models of LSD1 activity inhibition. Scale bars are equal to 10 µm. Statistical significance was analyzed at * p < 0.05 using ANOVA and post hoc analysis by Tukey’s test.