Figure 6.
The phenotype ofplants silencing miR825/825* target genes by B. cinerea infection. Three-week-oldplants of Arabidopsis Col-0 and miR825/825* target mutant lines (at5g38850, at3g04220) were pretreated with AR156 at 5 × 108 CFU/mL or 0.85% NaCl (the control) as a root drench. All plants were challenge-inoculated at 7 dpt as described above. (A) Disease symptoms developed at 2 dpi on leaves of each tested plant line. (B) Necrotic lesions were assessed at 2 dpi by measuring their average diameter on one leaf per plant, with a total of three plants assessed per sample. Two-way ANOVA showed significant effects of genotype (P < 0.01) and treatment (P < 0.01) and a significant interactive effect (P < 0.01). Note: ** P < 0.01. (C) In planta fungal growthin leaves of each tested plant line. The biomass of B. cinerea B1301 was measured as depicted above and indicated as BcActin/AtActin1. Two-way ANOVA indicated significant effects of genotype (P < 0.01) and treatment (P < 0.01) and a significant interactive effect (P < 0.01). Note: ** P < 0.01. The data are presented as mean ± SD from three biological replicates. The assays were repeated three times,yielding similar results.