Figure 5.

Formation of respiratory chain supercomplexes and in-gel activity assay of CI. Blue native PAGE and western blot detection of respiratory chain enzymes solubilized with digitonin. (A) Antibody against NDUFS1, a core subunit of the N-module in CI; (B) antibody against NDUFS2, a core subunit of the Q-module in CI; (C) antibody against NDUFB8, an accessary subunit of the P-module in CI; (D) antibody against UQCRC2, a core subunit of CIII; (E) antibody against SDHB, a subunit of complex II (CII), which serves as a loading control. (F) In-gel activity assay (IGA) of CI. Ctrl: control; MHM: mouse heart mitochondria, as molecular weight marker and positive control; SC: supercomplexes; III, complex III (CIII); IV, complex IV (CIV).