Table 3.
Splice variants of 7 VHA-H genes identified in 6 species of crops. Gene names are the same as those in Table 1. Alternative splicing sites occurring in the 5′ or 3′ UTRs are indicated in parentheses following the spliced exons.
| Gene | Transcript | Ensembl Transcript ID | Predicted Amino Acid Length (aa) | Spliced Exon | Status |
|---|---|---|---|---|---|
| GrVHA-H2 | GrVHA-H2.1 | KJB26715 | 481 | Wild type | |
| GrVHA-H2.2 | KJB26714 | 351 | Exon 1 | Alternative 5′ donor site | |
| Exon 3 | Alternative 3′ acceptor site | ||||
| Exons 4-7 | Exon skipping | ||||
| Exon 12 | Alternative 3′ acceptor site | ||||
| HvVHA-H | HvVHA-H.1 | HORVU2Hr1G042700.1 | 452 | Wild type | |
| HvVHA-H.2 | HORVU2Hr1G042700.2 | 452 | Exon 1 (5′ UTR) | Alternative 5′ donor site | |
| Exon 12 (3′ UTR) | Mutually exclusive exons | ||||
| HvVHA-H.3 | HORVU2Hr1G042700.3 | 452 | Exon 1 (5′ UTR) | Alternative 5′ donor site | |
| Exon 12 (3′ UTR) | Mutually exclusive exons | ||||
| HvVHA-H.4 | HORVU2Hr1G042700.4 | 450 | Exon 1 (5′ UTR) | Alternative 5′ donor site | |
| Exon 11 | Mutually exclusive exons | ||||
| Exon 12 (3′ UTR) | Exon skipping | ||||
| HvVHA-H.5 | HORVU2Hr1G042700.5 | 494 | Exon 1 (5′ UTR) | Alternative 5′ donor site | |
| Exon 10 | Alternative 3′ acceptor site | ||||
| Exons 11-12 (3′ UTR) | Exon skipping | ||||
| HvVHA-H.6 | HORVU2Hr1G042700.6 | 110 | Exon 1 (5′ UTR) | Exon skipping | |
| Exons 2-7 | Exon skipping | ||||
| Exons 10 | Alternative 3′ acceptor site | ||||
| Exons 11-12 (3′ UTR) | Exon skipping | ||||
| HvVHA-H.7 | HORVU2Hr1G042700.7 | 109 | Exon 1 (5′ UTR) | Exon skipping | |
| Exons 2-7 | Exon skipping | ||||
| Exon 11 (3′ UTR) | Mutually exclusive exons | ||||
| Exon 12 (3′ UTR) | Exon skipping | ||||
| StVHA-H2 | StVHA-H2.1 | PGSC0003DMT400029149 | 454 | Wild type | |
| StVHA-H2.2 | PGSC0003DMT400029148 | 454 | Exon 8 | Mutually exclusive exons | |
| Exon 12 (3′ UTR) | Exon skipping | ||||
| StVHA-H2.3 | PGSC0003DMT400029147 | 454 | Exon 12 (3′ UTR) | Exon skipping | |
| StVHA-H2.4 | PGSC0003DMT400029150 | 369 | Exon 9 (3′ UTR) | Mutually exclusive exons | |
| Exons 10-11 | Exon skipping | ||||
| Exons 12-13 (3′ UTR) | Exon skipping | ||||
| StVHA-H2.5 | PGSC0003DMT400029145 | 144 | Exons 1 (5′ UTR) | Exon skipping | |
| Exons 2-7 | Exon skipping | ||||
| Exon 8 | Alternative 5′ donor site | ||||
| Retained one exon between exons 8 and 9 | Exon skipping | ||||
| Exon 11 (3′ UTR) | Alternative 3′ acceptor site | ||||
| Exon 12, 13 (3′ UTR) | Exon skipping | ||||
| SiVHA-H | SiVHA-H.1 | KQL26169 | 458 | Wild type | |
| SiVHA-H.2 | KQL26168 | 404 | Exon 1 (5′ UTR) | Alternative 5′ donor site | |
| Exon 12 (3′ UTR) | Mutually exclusive exons | ||||
| TaVHA-H1 | TaVHA-H1.1 | TraesCS2A02G212100.2 | 452 | Wild type | |
| TaVHA-H1.2 | TraesCS2A02G212100.1 | 455 | Exon 12 | Mutually exclusive exons | |
| Exon 13 (3′ UTR) | Exon skipping | ||||
| ZmVHA-H1 | ZmVHA-H1.1 | Zm00001d006565_T002 | 452 | Wild type | |
| ZmVHA-H1.2 | Zm00001d006565_T001 | 379 | Exons 4-6 | Exon skipping | |
| Exon 7 | Alternative 5′ donor site | ||||
| ZmVHA-H1.3 | Zm00001d006565_T003 | 431 | Exons 1-2 (5′ UTR) | Exon skipping | |
| ZmVHA-H2 | ZmVHA-H2.1 | Zm00001d021721_T003 | 452 | Wild type | |
| ZmVHA-H2.2 | Zm00001d021721_T001 | 464 | Retained two exons between exons 3 and 4 | Exon skipping | |
| Exon 5 | Exon skipping | ||||
| ZmVHA-H2.3 | Zm00001d021721_T002 | 461 | Retained two exons between exons 3 and 4 | Exon skipping | |
| Exon 5 | Exon skipping | ||||
| Exon 6 | Alternative 3′ acceptor site | ||||
| Exon 7 | Alternative 5′ donor site | ||||
| ZmVHA-H2.4 | Zm00001d021721_T004 | 199 | Exons 1-2 (5′ UTR) | Exon skipping | |
| Retained exon between exons 2 and 3 (5′ UTR) | Exon skipping | ||||
| Introns 3-5 (5′ UTR) | Intron retention | ||||
| ZmVHA-H2.5 | Zm00001d021721_T005 | 431 | Exons 1-2 (5′ UTR) | Exon skipping |