Figure 4.

RBM20 transcriptional activity and activation of the mitogen-activated protein kinase (MAPK) signaling through insulin, T3, and Ang II. (A) Relative mRNA levels of RBM20 under different treatments; (B) Western blot analysis of hallmark proteins in the MAPK signaling cascade; (C–E) Quantification of relative phosphorylation levels of p38, ERK1/2, and JNK in comparison to their individual pho-antibodies. Glyceraldehyde-3-phosphate (GAPDH), protein loading control; NC, negative control; Mean ± SE (n = 3), * p < 0.05, ** p < 0.01, *** p < 0.001. Statistical significance was compared between different treatments and their individual negative controls.